DMEM Low Glucose Cell Culture Medium

How to Use DMEM in the Lab

Start by warming the medium to 37°C before adding it to your cultures. You'll need to supplement with 10% fetal bovine serum (FBS) or other required additives—this medium contains no proteins, lipids, or growth factors. Keep your cultures in a humidified incubator with 5–10% CO₂ to maintain the correct pH via the sodium bicarbonate buffer system. Avoid prolonged light exposure to protect the phenol red and other light-sensitive components.

Key Features of Hygia DMEM Low Glucose

• Proven Formulation: Works well with primary fibroblasts, neurons, glial cells, HUVECs, smooth muscle cells, and common lines like HeLa, 293, Cos-7, and PC-12.
• Sterile and Ready-to-Use: Supplied as a sterile liquid after 0.1 µm filtration and aseptic filling in a hundred-stage FFU. Filter integrity tests are performed before and after filtration.
• cGMP Compliant: Manufactured in a cGMP-compliant facility using water for injection. Each lot is tested for sterility, pH, osmolality, endotoxin, and cell culture performance.
• Supplement Required: No proteins, lipids, or growth factors are included; typically needs 10% FBS added before use.

What Is DMEM Cell Culture Medium?

DMEM (Dulbecco's Modified Eagle Medium) is a popular basal medium for growing various mammalian cells. It has four times the amino acids and vitamins found in Eagle's Minimal Essential Medium, making it nutrient-rich. This low glucose version (1.0 g/L) includes sodium pyruvate and uses phenol red as a pH indicator. The medium relies on a sodium bicarbonate buffer system (3.7 g/L) and needs a 5–10% CO₂ atmosphere to keep the pH in range.

Quality and Manufacturing Standards

Hygia Reagents maintains strict quality control. All raw materials pass rigorous testing. The final product is filtered through 0.1 µm sterile filters, with integrity tests verified. Each lot is checked for sterility, pH, osmolality, endotoxin, and cell culture performance to ensure consistent results.

FAQs About DMEM Cell Culture Medium

What cell types can I grow in this DMEM?

This low-glucose DMEM supports primary cells (fibroblasts, neurons, glial cells, HUVECs, smooth muscle cells) and established lines like HeLa, 293, Cos-7, and PC-12.

Do I need to add anything before use?

Yes, you'll need to add supplements—commonly 10% fetal bovine serum (FBS) and antibiotics if desired. It contains no proteins, lipids, or growth factors.

Why is CO₂ necessary?

The sodium bicarbonate buffer system requires 5–10% CO₂ to maintain the correct pH for cell growth.

For research use only. Not for diagnostic or therapeutic use.